Composite

Part:BBa_K3440012:Design

Designed by: Julie Cordier, Aditi Kallai   Group: iGEM20_Stockholm   (2020-10-23)


pRhl promoter with GFP activated by RhlR expression and AHL


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 880
    Illegal BamHI site found at 301
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 776
    Illegal BsaI.rc site found at 1754


Design Notes

This part was designed to show that Prhl promoter can be activated in the presence of AHL and RhlR expression. Moderate RBS was used. GFP was used as a reporter of Prhl activation Myc-tag was used as a reporter of RhlR expression.

Source

RhlR from Pseudomonas aeruginosa

References